Anti-AAR2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y312

Core Product Specifications and Parameters

Biological Background: AAR2 Function and Localization

• Protein AAR2 homolog, also known as AAR2 splicing factor homolog (gene AAR2/C20orf4), is a conserved component of the U5 small nuclear ribonucleoprotein (snRNP) complex.
• AAR2 is essential for spliceosome assembly and pre-mRNA splicing, directly contributing to accurate mRNA processing.
• The protein is categorized under mRNA processing and spliceosome pathways, with involvement in key steps of intron removal.
• Post-translational modifications include acetylation, and AAR2 has been detected in proteomics identification studies.
• Its molecular weight is approximately 43.5 kDa (UniProt calculated mass: 43472 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 1-250 of human AAR2) targets the N-terminal region; this antibody may detect both full-length and N-terminal fragments in Western blot.
• For Western blot, a band near 43 kDa is expected in lysates from human or mouse cells expressing AAR2. Run appropriate controls and consider loading amounts.
• In IF/ICC, optimization of fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) is recommended to balance epitope accessibility and structure preservation.
• ELISA and other immunoassays can be performed using standard protocols; titrate the antibody to achieve optimal signal-to-noise.
• Validate reactivity in your specific sample system, as expression levels may vary across cell types and conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research labs by providing one-stop access to validated IVD raw materials, including monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-AAR2 antibody supports investigations into spliceosome dynamics and mRNA processing, and we offer comprehensive supply solutions with technical support across all development stages, from concept to clinic.