Anti-AANAT Rabbit Polyclonal Antibody for WB and ELISA - Q16613

Core Product Specifications and Parameters

Biological Background: AANAT Function and Localization

• Serotonin N-acetyltransferase (AANAT), also known as aralkylamine N-acetyltransferase (AA-NAT) or SNAT, is encoded by the AANAT gene.
• It catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in melatonin biosynthesis, thereby controlling the night/day rhythm of melatonin production in the pineal gland.
• AANAT belongs to the acyltransferase and transferase families and plays a key role in biological rhythms.
• The enzyme is primarily localized in the cytoplasm.
• It is highly expressed in the pineal gland and, at lower levels, in the retina, with weak expression in several brain regions and the pituitary gland.
• The protein undergoes phosphorylation and alternative splicing, and its three-dimensional structure has been determined.
• The molecular weight is approximately 23.3 kDa (UniProt), consistent with the observed band at 23 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (aa 1–207), which may elicit antibodies recognizing both native and denatured epitopes; consider this when optimizing sample preparation.
• For Western blotting, validate the antibody with your specific sample system; starting with a standard dilution range (e.g., 1:500–1:2000) under reducing conditions is suggested.
• For ELISA, the polyclonal nature can enhance capture/detection sensitivity; however, assay conditions should be individually optimized.
• Cross-reactivity with other species is not tested; if applied beyond human samples, verify reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-AANAT antibody empowers studies in melatonin biosynthesis and circadian rhythm disorders. We offer validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development and commercialization.