Anti-AAMP Monoclonal Antibody for WB, IF/ICC, ELISA - Q13685

Core Product Specifications and Parameters

Biological Background: AAMP Function and Localization

• AAMP (angio-associated migratory cell protein) is a ~47 kDa protein that contains WD repeats, suggesting a role in coordinating multi-protein complex assemblies.
• Plays a functional role in angiogenesis and cell migration; in smooth muscle cells, it may mediate migration through the RhoA signaling pathway.
• Localizes to both the cell membrane and the cytoplasm, consistent with its involvement in dynamic cellular processes.
• Widely expressed at the protein level, with notable presence in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics.
• Classified as a phosphoprotein, indicating potential regulation by phosphorylation.
• Associated with developmental processes and differentiation, based on UniProtKB keywords.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human, mouse, or rat endothelial or tumor cell lines; expect a specific band near 47 kDa.
• In immunofluorescence/immunocytochemistry, permeabilization may be necessary to detect cytoplasmic AAMP; validate in appropriate positive controls such as HUVECs or A2058 melanoma cells.
• When using ELISA, pair this antibody with a suitable detection system and optimize coating and blocking conditions for your sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-AAMP monoclonal antibody as part of its comprehensive IVD raw-material portfolio, supporting angiogenesis and cell migration research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic development from concept to clinic. Partner with CamelBio for reliable, one-stop access to the critical reagents you need.