Anti-AAK1 Rabbit Polyclonal Antibody for WB, ELISA - Q2M2I8

Core Product Specifications and Parameters

Biological Background: AP2-associated protein kinase 1 Function and Localization

• AAK1 (Adaptor-associated kinase 1) exists as at least two isoforms (isoform 1 and isoform 2), both displaying similar kinase activity towards AP2M1. Related references: PMID:17494869

• Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the AP-2 adaptor complex, enhancing cargo binding; additionally controls AP-2 subunit phosphorylation and localization. Exhibits a preference for threonine residue phosphorylation. Related references: PMID:11877457, PMID:11877461, PMID:12952931, PMID:18657069

• Phosphorylates NUMB, directing its localization to endosomes, and binds to and stabilizes the activated NOTCH1 receptor, modulating its transcriptional activity. Related references: PMID:18657069, PMID:21464124

• Localizes to the cell membrane, clathrin-coated pits, and presynaptic compartments.

• Expressed in brain (isoform 1 predominant), heart, and liver.

• Facilitates viral entry of hepatitis C, Ebola, and Dengue viruses through the endocytic pathway.

• Features include acetylation and phosphorylation modifications; classified within the ATP-binding, nucleotide-binding, and serine/threonine-protein kinase families.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 450–550) of human AAK1; consider verifying sequence homology across species and isoforms to predict cross-reactivity.
• For Western blotting with a predicted molecular weight of ~103 kDa, use optimized gel percentage, extended transfer, and high-molecular-weight markers.
• ELISA may be applied for detection or relative quantitation; include proper negative controls and validate reactivity in the sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop procurement platform for IVD raw materials, supporting manufacturers and laboratories with everything from antibody pairs to rare target reagents. For researchers investigating AAK1 in endocytosis or viral entry, we supply optimized polyclonal and monoclonal antibodies alongside comprehensive technical and sourcing services. Contact our team to streamline your complete raw-material supply chain.