Anti-AADAT Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8N5Z0

Core Product Specifications and Parameters

Biological Background: AADAT Function and Localization

• AADAT (Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a pyridoxal phosphate-dependent aminotransferase encoded by the AADAT gene, also known as KAT2 or KYAT2.
• The enzyme exhibits broad substrate specificity, catalyzing transamination of aminoadipate, kynurenine, methionine, and glutamate, with additional activity toward tryptophan, aspartate, and hydroxykynurenine.
• It utilizes various oxo-acid amino-group acceptors, preferring 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate, and alpha-oxo-gamma-methiol butyric acid, and can employ glyoxylate in vitro.
• AADAT localizes primarily to the mitochondrion, consistent with its role in amino acid metabolism.
• Expression is highest in liver, with detectable levels in heart, brain, kidney, pancreas, prostate, testis, and ovary.
• The protein undergoes alternative splicing and acetylation, and its 3D structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 34-429 of human AADAT, covering most of the mature protein; consider sequence alignment for cross-species reactivity assessment.
• For Western blot, a band around 47 kDa is expected; use appropriate mitochondrial protein extraction protocols if enriching for the target.
• For IHC-P and IF/ICC, include mitochondrial co-staining markers to confirm subcellular localization.
• ELISA applications may require optimization of coating antigen and detection system for quantitative detection.
• Always validate the antibody in your specific sample type and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Our portfolio includes this anti-AADAT polyclonal antibody, suitable for research into mitochondrial aminotransferase functions and related disorders. Whatever your diagnostic development needs, CamelBio provides reliable access to high-quality materials and technical support.