Anti-AADAT Rabbit Polyclonal Antibody for WB, ELISA - Q8N5Z0

Core Product Specifications and Parameters

Biological Background: AADAT Function and Localization

• AADAT, also known as kynurenine/alpha-aminoadipate aminotransferase mitochondrial, is encoded by the AADAT gene and alternatively named KAT2, KYAT2. It is a pyridoxal phosphate-dependent aminotransferase.
• The enzyme exhibits broad substrate specificity, catalyzing transamination of aminoadipate, kynurenine, methionine, and glutamate. It also acts on tryptophan, aspartate, and hydroxykynurenine, accepting various oxo-acids with a preference for 2-oxoglutarate.
• AADAT plays a key role in amino acid metabolism, converting kynurenine to kynurenic acid, a neuroprotective metabolite, linking it to neurodegenerative disease research.
• Subcellularly, AADAT is localized to the mitochondrion, consistent with its role in mitochondrial amino acid degradation and energy metabolism.
• Tissue expression is highest in liver, with detectable levels in heart, brain, kidney, pancreas, prostate, testis, and ovary, suggesting widespread metabolic functions.
• The protein undergoes alternative splicing and acetylation, with a transit peptide for mitochondrial import, and has been identified in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen was designed against a recombinant fusion protein spanning amino acids 34-429 of human AADAT, covering the majority of the mature protein. For optimal detection, consider using this antibody in WB and ELISA, as validated in relevant applications.
• Given cross-reactivity with rat, this antibody may be suitable for comparative studies between human and rat models. Validate performance in the specific tissue or cell line of interest.
• The predicted molecular weight of AADAT is ~47 kDa; confirm band size under your experimental conditions, as post-translational modifications or alternative splicing may affect migration.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research labs with reliable reagents, technical services, and consulting—covering every stage from concept to clinic. For your AADAT-related projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets, helping you accelerate assay development and biomarker research.