Anti-AADAC Polyclonal Antibody for WB, ELISA - P22760

Core Product Specifications and Parameters

Biological Background: AADAC Function and Localization

• AADAC (arylacetamide deacetylase, also designated DAC or CES5A1) is a serine hydrolase that belongs to the carbohydrate esterase family.
• It displays cellular triglyceride lipase activity in the liver, hydrolyzing newly formed triglyceride stores to increase intracellular fatty acid levels and contributing to very low-density lipoprotein (VLDL) assembly.
• AADAC exhibits serine esterase activity and deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to primary arylamide compounds with enhanced toxicity.
• The protein is anchored to the endoplasmic reticulum and microsome membranes via a single-pass transmembrane domain.
• At the protein level, AADAC is detected in the liver, and it is predominantly expressed in liver, small intestine, colon, adrenal gland, and bladder tissues.
• Post‑translational modifications include N‑linked glycosylation and the presence of a disulfide bond, which are important for proper folding and stability.

Experimental Guidance and Technical Tips

• The immunogen spans the C‑terminal region (amino acids 170–399); the resulting polyclonal antibodies may recognize both full‑length and truncated AADAC forms. Validate specificity using appropriate positive and negative controls in the sample system of interest.
• For Western blot, expect a band around 46 kDa in human and mouse liver lysates. Use freshly prepared samples with protease inhibitors to minimize degradation.
• In ELISA, perform a titration series to determine the optimal antibody dilution. Cross‑reactivity with mouse AADAC enables comparative studies across species.
• Given the membrane association of AADAC, enrichment of microsomal fractions or use of liver membrane preparations can improve detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of high‑quality IVD raw materials, including validated polyclonal antibodies like this anti‑AADAC reagent. Supporting investigations into lipid metabolism and xenobiotic processing, we offer ready‑to‑use antibody pairs, bulk‑scale ancillary reagents, and custom sourcing for rare‑target biomaterials to accelerate your assay development from concept to application.