Anti-AAAS Rabbit Polyclonal Antibody for WB and ELISA - Q9NRG9

Core Product Specifications and Parameters

Biological Background: Aladin (AAAS) Function and Localization

• Aladin (AAAS) is a WD repeat-containing protein with a predicted molecular weight of ~60 kDa, and multiple isoforms are produced by alternative splicing.
• Aladin is essential for normal development of the peripheral and central nervous system. Related references: PMID:11062474, PMID:11159947, PMID:16022285
• Aladin is required for correct spindle formation and timely chromosome alignment during mitosis, mediated by proper localization of AURKA and NUMA1. Related references: PMID:26246606
• Aladin predominantly localizes to the nuclear pore complex and nuclear envelope, and additionally associates with spindle poles during cell division.
• Widely expressed, Aladin is particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures, and fetal lung. Related references: PMID:11159947, PMID:16022285
• Aladin undergoes acetylation and phosphorylation and is implicated in mRNA and protein transport through the nuclear pore complex.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 287–546) spans the C-terminal region of human Aladin, which may enhance detection of the full-length protein in Western blotting. A specific band at ~60 kDa is expected in human cell or tissue lysates. Additional bands may arise from isoforms or post‑translational modifications.
• For ELISA applications, consider using the antibody as a capture or detection reagent after appropriate titration against the recombinant immunogen or native Aladin in relevant sample matrices.
• Since the antibody is polyclonal, lot‑to‑lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical support for diagnostic development, from concept to clinic. As your one‑stop sourcing partner, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, ancillary reagents, and can source rare targets to accelerate your AAAS‑related research in nuclear transport, neurobiology, and cell cycle studies.