Anti-A1CF (APOBEC1 Complementation Factor) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NQ94

Core Product Specifications and Parameters

Biological Background: A1CF Function and Localization

• A1CF (APOBEC1 complementation factor), also designated ACF, ASP, ACF64, ACF65, or APOBEC1CF, is a 65 kDa RNA-binding protein that serves as an essential component of the apolipoprotein B (APOB) mRNA editing complex.
• It functions as the RNA-binding subunit that docks the catalytic deaminase APOBEC1 to the APOB mRNA, enabling site-specific cytidine-to-uridine editing that converts a glutamine codon (CAA) into a premature stop codon (UAA).
• The editing complex also stabilizes the edited APOB mRNA by protecting it from nonsense-mediated decay, thereby ensuring production of the truncated APOB protein isoform.
• Subcellularly, A1CF localizes to the nucleus, endoplasmic reticulum, and cytoplasm, suggesting diverse roles in mRNA processing and transport.
• It is widely expressed across tissues, with the highest levels observed in brain, liver, pancreas, colon, and spleen.
• Post-translational modifications include phosphorylation, as indicated by proteomics identification and database annotations.
• Additionally, A1CF undergoes alternative splicing and contains repeat domains, contributing to its functional versatility as an RNA-binding phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–120 of human A1CF, a region likely to be exposed in the native protein; consider this when designing epitope-based detection strategies.
• For Western blot (WB), the predicted molecular weight is ~65 kDa; validate detection in human, mouse, or rat tissue lysates such as liver or brain where A1CF is highly expressed.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using formalin-fixed paraffin-embedded sections; tissues with high endogenous A1CF (e.g., liver, pancreas) are suitable positive controls.
• In ELISA, confirm specificity by testing against recombinant A1CF or tissue extracts, and consider pairing with a monoclonal capture antibody if sandwich formats are desired.
• As a polyclonal antibody, expect some lot-to-lot variation; we recommend confirming performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material procurement. This anti-A1CF antibody supports research and assay development in apolipoprotein biology and mRNA editing pathways. Whether you require single reagents or integrated supply solutions, CamelBio bridges your concept-to-clinic needs with reliable B2B support.