Anti-A1BG Polyclonal Antibody for WB, ELISA - P04217

Core Product Specifications and Parameters

Biological Background: A1BG Function and Localization

• Alpha-1B-glycoprotein (A1BG) is a secreted glycoprotein of approximately 54 kDa, containing immunoglobulin-like domains and internal repeats, thus belonging to the immunoglobulin superfamily.
• The A1BG gene generates multiple transcripts through alternative splicing, resulting in distinct protein isoforms.
• A1BG is predominantly expressed and secreted into blood plasma, as evidenced by its signal peptide and tissue-specificity data.
• Post-translational modifications include N-linked glycosylation and intramolecular disulfide bonds, which are typical for secreted glycoproteins.
• Its presence in the human proteome has been confirmed by both direct protein sequencing and mass spectrometry-based proteomics.
• The precise biological function of A1BG remains poorly understood; however, its structural resemblance to immunoglobulins suggests a potential role in immune-related processes.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 236–495 of human A1BG (NP_570602.2). For applications requiring cross-species detection, align this region with mouse/rat orthologs to inform expected reactivity.
• In Western blotting, the predicted mass is ~54 kDa. Use plasma or serum as a positive control and ensure proper normalization to confirm specific band detection.
• For ELISA development, the polyclonal nature of this antibody may offer broad epitope coverage. Test both direct and indirect formats and optimize coating antigen concentrations.
• Cross-reactivity with mouse and rat A1BG is indicated; however, sequence conservation in the immunogen region should be verified for quantitative comparative studies.
• Maintain cold-chain handling as per shipping conditions (ice bag) to preserve antibody activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, and this A1BG rabbit polyclonal antibody exemplifies our focus on plasma protein research and diagnostic assay development. We offer validated antibody pairs, bulk ancillary reagents, and access to rare targets, supporting your workflow from feasibility through commercialization. Reach out to leverage our integrated sourcing services for your next project.