Anti-α-Synuclein Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein is a neuronal protein primarily expressed at presynaptic terminals in the central nervous system, particularly in the brain.
• It plays a key role in synaptic vesicle trafficking and neurotransmitter release by enhancing vesicle priming, fusion, and dilation of exocytotic fusion pores. Related references: PMID:28288128, PMID:30404828
• Acts as a molecular chaperone in a multimeric membrane-bound state, assisting the folding of SNARE proteins, crucial for sustaining normal SNARE-complex assembly during aging. Related references: PMID:20798282
• Modulates dopamine neurotransmission by associating with the dopamine transporter (DAT1) and regulating its activity. Related references: PMID:26442590
• Mechanistically, increases local Ca2+ release from microdomains to enhance ATP-induced exocytosis. Related references: PMID:30404828
• Found in the cytoplasm, membrane, nucleus, synapse, and secreted; also present in cell projections like axons.
• Keywords indicate involvement in neurodegeneration, Parkinson disease, Alzheimer disease, amyloid formation; undergoes acetylation, phosphorylation, ubiquitination, and metal binding (copper).

Experimental Guidance and Technical Tips

• For Western blot, the detected band is ~14 kDa; use appropriate gel percentage and transfer conditions for low-molecular-weight proteins.
• For immunohistochemistry and immunofluorescence, optimize antigen retrieval and fixation methods for the specific tissue and target accessibility, as α-synuclein is expressed in presynaptic terminals.
• Since the immunogen corresponds to the full-length protein, the antibody may recognize total α-synuclein; consider potential post-translational modification detection requirements.
• For ELISA, consider validating capture/detection antibody pairs if used in sandwich format.
• Cross-reactivity with mouse and rat supports use in rodent models; validate in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For alpha-synuclein research and related neurodegenerative disease applications, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your supply chain from discovery to diagnostic development.