Anti-α-Synuclein Monoclonal Antibody for WB - P37840

Core Product Specifications and Parameters

Biological Background: α-Synuclein Function and Localization

• Alpha-synuclein (SNCA) is a small presynaptic protein belonging to the synuclein family, encoded by the SNCA gene (also known as NACP, PARK1/4). It is highly expressed in brain, particularly at presynaptic terminals.
• It plays key roles in synaptic vesicle trafficking and neurotransmitter release, acting as a monomer to enhance vesicle priming, fusion, and dilation of exocytotic fusion pores. Related references: PMID:28288128, PMID:30404828
• Within the synapse, alpha-synuclein functions as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of SNARE proteins at the presynaptic membrane in conjunction with cysteine string protein-α, which is important for sustaining SNARE-complex assembly during aging. Related references: PMID:20798282
• It also modulates dopamine neurotransmission by associating with the dopamine transporter (DAT1) and regulating its activity. Related references: PMID:26442590
• Subcellularly, alpha-synuclein localizes to the cytoplasm, membrane, nucleus, synapse, and is also secreted and present in cell projections such as axons.
• Tissue specificity: Highly expressed in presynaptic terminals in the central nervous system, principally in brain.
• Alpha-synuclein is implicated in neurodegenerative disorders like Alzheimer’s and Parkinson’s disease; it undergoes alternative splicing and various post‑translational modifications, including phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen (a.a. 59–140) covers the C‑terminal region of α‑synuclein, which is retained in most isoforms and aggregated forms. Consider this when interpreting results across different sample types.
• For Western blotting, a band at approximately 14 kDa is expected. Optimize sample preparation (e.g., avoiding boiling, using mild detergents) to preserve native aggregation states if studying oligomers.
• In IF/ICC and IF‑P, validate the antibody in neuronal cell lines or brain tissue sections known to express α‑synuclein; co‑staining with synaptic markers may help confirm localization.
• ELISA protocols should include appropriate controls and consider the assay format (direct, sandwich) to detect α‑synuclein in biological fluids, keeping in mind the protein’s tendency to aggregate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one‑stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and laboratories with high‑quality reagents and technical support from concept to clinic. Through our extensive network, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing—helping you accelerate assays for neurodegenerative disease markers including α‑synuclein in Parkinson’s and Alzheimer’s research.