Anti-α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P62736

Core Product Specifications and Parameters

Biological Background: α-Smooth Muscle Actin (ACTA2) Function and Localization

• ACTA2 encodes aortic smooth muscle actin (alpha-actin-2), a highly conserved cytoskeletal protein involved in cell motility and structural integrity, expressed ubiquitously in eukaryotic cells.
• As a member of the actin family, ACTA2 is a key component of the contractile apparatus in vascular smooth muscle cells, contributing to vessel tone and blood pressure regulation.
• Mutations in ACTA2 are associated with aortic aneurysm (disease variant), underscoring its critical role in maintaining aortic wall stability.
• The protein localizes predominantly to the cytoplasm and cytoskeleton, where it polymerizes into microfilaments essential for cell shape, division, and migration.
• ACTA2 undergoes post-translational modifications including acetylation, methylation, and oxidation, which may regulate actin dynamics and interactions with binding partners.
• Alternative names include Alpha-actin-2 and Cell growth-inhibiting gene 46 protein, reflecting its roles beyond structural support in cell growth inhibition.

Experimental Guidance and Technical Tips

• The immunogen is an N-terminal peptide (aa 1-100) of human ACTA2; thus, the antibody may detect total ACTA2 protein in your samples.
• For tissue-specific analysis, consider validating in smooth muscle-rich tissues (e.g., aorta, bladder) and using positive controls such as human, mouse, or rat smooth muscle cell lysates.
• In Western blotting, a band at approximately 42 kDa is expected; optimize loading controls and antibody dilution for your system.
• Cross-reactivity with human, mouse, and rat is reported; due to high sequence conservation, the antibody may also recognize ACTA2 from other species—validate if required.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), test antigen retrieval methods and antibody concentrations on known positive tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined sourcing channel for IVD raw materials, including validated antibodies like this anti-ACTA2 polyclonal antibody. We offer procurement support for antibody pairs, bulk ancillary reagents, and rare target raw materials, helping accelerate your smooth muscle and cytoskeleton research projects. Reach out to explore how our one-stop supply solutions can support your workflow from concept to clinic.