Anti-α-Smooth Muscle Actin (ACTA2) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P62736

Core Product Specifications and Parameters

Biological Background: α-Smooth Muscle Actin Function and Localization

• ACTA2 encodes actin, aortic smooth muscle (also known as alpha-actin-2 or cell growth-inhibiting gene 46 protein), a member of the highly conserved actin protein family involved in cell motility.
• Actins are ubiquitously expressed in all eukaryotic cells and participate in various types of cell motility processes.
• The protein localizes to the cytoplasm and is a major component of the cytoskeleton.
• Post-translational modifications include acetylation, methylation, and oxidation, which may regulate its function.
• ACTA2 is associated with disease variants, including aortic aneurysm, underlining its critical role in vascular smooth muscle integrity.
• ATP-binding and hydrolase activity are key functional features, reflecting its role in cytoskeletal dynamics.

Experimental Guidance and Technical Tips

• The immunogen was designed from the N-terminal region (aa 1–100) of human ACTA2; sequence alignment with other actin isoforms is recommended to assess isoform specificity.
• For Western blotting, a band around 42 kDa is expected; validate the antibody in lysates from smooth muscle-containing tissues like aorta or bladder.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), ACTA2 exhibits filamentous cytoplasmic staining; suitable positive controls include smooth muscle cell lines or tissue sections.
• ELISA can be used for quantitative detection; consider coating the plate with the recombinant immunogen or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For smooth muscle and cytoskeleton research, our anti-ACTA2 polyclonal antibody is a reliable raw material solution, backed by our one-stop technical support from concept to clinic. Contact our team to discuss your custom sourcing needs and supply chain optimization.