Anti-α-Smooth Muscle Actin (ACTA2) Rabbit Monoclonal Antibody for WB, ELISA - P62736

Core Product Specifications and Parameters

Biological Background: α-Smooth Muscle Actin (ACTA2) Function and Localization

• ACTA2 encodes alpha-smooth muscle actin, a member of the actin family, highly conserved and ubiquitously expressed in all eukaryotic cells.
• It is a major component of the contractile apparatus in smooth muscle cells, contributing to cell motility and structural integrity.
• The gene is located on chromosome 10 and is assigned alternate names including Alpha-actin-2 and cell growth-inhibiting gene 46 protein.
• ACTA2 localizes predominantly to the cytoplasm, integrating into the cytoskeleton and stress fibers.
• Post-translational modifications such as acetylation, methylation, and oxidation are noted, reflecting dynamic regulation.
• Mutations in ACTA2 are associated with thoracic aortic aneurysms and dissections, a disease variant keyword.
• The protein has a molecular weight of approximately 42 kDa (predicted 42009 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100); consider that the epitope is likely linear and conserved across human, mouse, and rat.
• For Western blot, expect a band around 42 kDa; validate in your specific smooth muscle lysate and use appropriate loading controls.
• The antibody may be suitable for ELISA detection of the immunogen peptide or native protein; optimize coating and detection conditions.
• Due to high conservation of actin, confirm band specificity by using actin isoform-specific controls or siRNA knockdown.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated IVD raw-material partner, CamelBio offers one-stop access to validated antibodies like this anti-ACTA2 monoclonal antibody, supporting smooth muscle and cytoskeleton research. Beyond individual reagents, we supply bulk custom antibody development, optimized antibody pairs, and a full range of ancillary raw materials to accelerate your diagnostic assay pipeline from concept to clinic.