Anti-α-Actin-1 (α-SMA) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P68133

Core Product Specifications and Parameters

Biological Background: α-Actin-1 Function and Localization

• Alpha-actin-1 (ACTA1) encodes actin, alpha skeletal muscle, a highly conserved cytoskeletal protein involved in cell motility and structural integrity.
• As a core component of the sarcomere thin filament in skeletal muscle, it plays a critical role in muscle contraction and force generation.
• ACTA1 belongs to the actin gene family, which includes six isoforms expressed in a tissue-specific manner; alpha-actin-1 is predominantly expressed in skeletal muscle.
• The protein localizes to the cytoplasm, integrating into the cytoskeleton and contributing to intracellular transport and cellular architecture.
• Key post-translational modifications include acetylation, methylation, and oxidation, which modulate its polymerization dynamics and interactome.
• Disease-associated variants in ACTA1 are linked to nemaline myopathy and other congenital myopathies, highlighting its essential role in muscle function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved C-terminal region (aa 278-377) of human ACTA1, enabling broad species reactivity (human, mouse, rat).
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency for reproducible assays across WB, IF-P, IHC-P, and ELISA.
• For Western blotting, expect a band at approximately 42 kDa; optimize loading controls and antibody dilution for your sample type.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), include appropriate controls to confirm muscle-specific staining patterns.
• Validate antibody performance in your experimental system, as the predicted cross-reactivity may vary with sample preparation and fixation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies the procurement of high-quality IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-α-Actin-1 (α-SMA) antibody supports research and diagnostic development in skeletal muscle biology, myopathy studies, and cytoskeleton-related diseases. Whether you need bulk supplies or custom sourcing solutions, CamelBio bridges your concept-to-clinic journey with dependable supply chain and technical consultation.