Anti-67kDa Laminin Receptor (RPSA) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P08865

Core Product Specifications and Parameters

Biological Background: 67kDa Laminin Receptor (RPSA) Function and Localization

• The protein encoded by RPSA (Gene ID: 3921) is officially designated as small ribosomal subunit protein uS2 and is also known as 37 kDa laminin receptor precursor (37LRP), 67 kDa laminin receptor (67LR), LBP/p40, and LRP/LR, among multiple aliases.
• It is essential for the assembly and stability of the 40S ribosomal subunit, processing 20S pre-rRNA to mature 18S rRNA, and simultaneously functions as a high-affinity cell surface receptor for laminin, mediating cell adhesion to the basement membrane and activating downstream signaling pathways.
• The 33 kDa ribosomal protein SA can form a mature ~67 kDa laminin receptor via dimerization or post-translational modifications, and it influences cell fate determination and tissue morphogenesis.
• Subcellular localization includes the cell membrane, cytoplasm, and nucleus, consistent with its dual roles in ribosome biogenesis and laminin signaling.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitination (UniProt keywords: Acetylation, Phosphoprotein, Isopeptide bond, Ubl conjugation).
• During microbial infection, RPSA acts as a host cell receptor for multiple viruses, including adeno-associated viruses 2,3,8,9, dengue virus, Sindbis virus, Venezuelan equine encephalitis virus, pathogenic prion protein, and certain bacteria.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 196-295) of human laminin receptor, which may allow detection of both the 33 kDa precursor and the 67 kDa mature form. Consider validating the antibody in lysates of laminin-responsive cell lines (e.g., HeLa, MDA-MB-231) for expected band patterns.
• For Western blotting, be aware that the target protein runs at approximately 33 kDa under denaturing conditions, while the 67 kDa form may be observed under non-reducing or cross-linking conditions.
• In IF/ICC experiments, expect staining at the cell membrane, cytoplasm, and nucleus, reflecting the protein’s multifunctional localization. Optimize fixation and permeabilization to preserve the epitope.
• For ELISA, pair this monoclonal antibody with a compatible detection reagent and validate sensitivity in the relevant sample matrix, especially if targeting the 67 kDa laminin receptor form.

CamelBio: Your One-Stop Sourcing Bridge

Leverage CamelBio’s comprehensive sourcing services to integrate this anti-67kDa laminin receptor rabbit monoclonal antibody into your diagnostic or research workflows. As a one-stop provider of IVD raw materials, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, with expertise in rare target procurement. Whether advancing cancer biomarker studies or developing infectious disease assays, our team ensures reliable, end-to-end material support from concept to clinic.