Anti-67kDa Laminin Receptor Polyclonal Antibody for WB, IF/ICC, ELISA - P08865

Core Product Specifications and Parameters

Biological Background: 67kDa Laminin Receptor Function and Localization

• The 67kDa laminin receptor (67LR), also known as 40S ribosomal protein SA (RPSA) or small ribosomal subunit protein uS2, is a multifunctional protein that exists as a 37 kDa precursor (37LRP) and a mature 67 kDa form which is thought to arise from homo-dimerization or post-translational modification.
• As a ribosomal protein, it is essential for the assembly and stability of the 40S ribosomal subunit and participates in the processing of 20S rRNA-precursor to mature 18S rRNA, thereby playing a critical role in ribosome biogenesis and translation.
• On the cell surface, 67LR functions as a laminin receptor, mediating cell adhesion to the basement membrane and activating intracellular signaling pathways that influence cell fate determination and tissue morphogenesis.
• The protein is localized to the cell membrane, cytoplasm, and nucleus, reflecting its diverse roles in ribosomal assembly, cell adhesion, and nuclear functions.
• 67LR acts as a host receptor for multiple viruses including adeno-associated viruses 2, 3, 8, and 9, dengue virus, Sindbis virus, and Venezuelan equine encephalitis virus, as well as for pathogenic prion protein and bacteria, highlighting its significance in infectious disease.
• Post-translational modifications such as acetylation, phosphorylation, and isopeptide bond formation (sumoylation) regulate its activity, and it acts as a substrate of PPP1CA via PPP1R16B.
• The protein is broadly conserved and classified under the ribosomal protein uS2 family, with disease variants associated with it as indicated by the UniProt keyword 'Disease variant'.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein spanning amino acids 1-295, corresponding to the full-length 37 kDa laminin receptor precursor. This antibody is expected to detect both the 37 kDa precursor and the 67 kDa mature form, with the 37 kDa band being the primary monomeric species observed in denaturing Western blot.
• Due to its ability to recognize the native form, this polyclonal antibody is suitable for applications including Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For WB, consider using reducing and non-reducing conditions to distinguish monomeric and dimeric forms.
• Cross-reactivity has been validated with human, mouse, and rat samples. For experiments in other species, verify sequence homology as the epitope is located within the highly conserved N-terminal region.
• When performing IF/ICC, optimize permeabilization and fixation protocols to detect the protein in its membranous, cytoplasmic, and nuclear compartments, as its distribution is cell-type dependent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and research institutes from concept to clinic. This anti-67 kDa laminin receptor polyclonal antibody addresses critical needs in oncology, infectious disease, and ribosomal biology research, from studying viral entry mechanisms to investigating tumor cell adhesion and metastasis. With our integrated supply chain and technical consultancy, we empower rapid and reliable development of next-generation diagnostics.