Anti-5HT7 Receptor Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P34969

Core Product Specifications and Parameters

Biological Background: 5HT7 Receptor Function and Localization

• The 5HT7 receptor (5-hydroxytryptamine receptor 7, 5-HT-X, serotonin receptor 7) is encoded by the HTR7 gene and belongs to the G protein-coupled receptor family.
• It functions as a receptor for serotonin, a biogenic hormone acting as a neurotransmitter, hormone, and mitogen. Ligand binding triggers G(s)-protein coupling and activates adenylate cyclase, elevating intracellular cAMP. Related references: PMID:35714614, PMID:8226867
• The receptor is localized at the cell membrane and contains seven transmembrane helices, characteristic of GPCRs.
• Predominant isoform is highly expressed in the spleen, caudate, and hippocampus, with lower levels in other tissues. Alternative splicing generates additional isoforms.
• Post-translational modifications include glycosylation, palmitoylation, and disulfide bond formation, which may influence receptor trafficking and signaling.
• The observed molecular weight is approximately 54 kDa, consistent with the calculated mass of 53.6 kDa plus modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the N-terminal region (amino acids 1–100), which may recognize multiple isoforms; validate specificity in your target tissue.
• For Western blotting, a major band around 54 kDa is expected; consider that glycosylation or other modifications may shift the band slightly.
• For immunofluorescence on fixed/permeabilized cells, optimize permeabilization to ensure antibody access to the intracellular N-terminal epitope.
• ELISA protocols should use appropriate coating buffers and antigen concentrations; cross-reactivity with mouse and rat suggests utility across species, but confirm with endogenous controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This Anti-5HT7 Receptor Rabbit mAb is well-suited for neuroscience and GPCR signaling studies, and CamelBio can assist in sourcing rare targets or scaling up production to meet your clinical or research demands. From initial concept to clinical application, CamelBio streamlines your supply chain with technical and procurement expertise.