Anti-53BP1 Rabbit Monoclonal Antibody for IHC-P/IF/ICC/ELISA - Q12888

Core Product Specifications and Parameters

Biological Background: 53BP1 Function and Localization

• Plays a key role in repair of double-strand DNA breaks by promoting non-homologous end joining and counteracting BRCA1-mediated homologous recombination. Related references: PMID:22553214, PMID:23333306, PMID:23727112
• Involved in DNA damage response, telomere dynamics, and class-switch recombination during antibody genesis. Related references: PMID:12364621, PMID:17190600, PMID:21144835
• Phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSB sites. Related references: PMID:28241136
• Recruited to DSB sites by binding histone H2A monoubiquitinated at Lys-15 and histone H4 dimethylated at Lys-20. Related references: PMID:17190600, PMID:23760478, PMID:27153538
• Required for immunoglobulin class-switch recombination. Related references: PMID:23345425
• Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1. Related references: PMID:23727112
• Subcellular localization: Nucleus, chromosome, centromere, kinetochore.
• Subject to post-translational modifications including phosphorylation, methylation, and ubiquitylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100); the antibody may recognize both full-length and N-terminal isoforms.
• For IHC-P, standard antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer) may be employed; validate in the relevant sample system.
• For IF/ICC, fix and permeabilize cells appropriately; consider testing multiple fixation protocols.
• For ELISA, pair with a suitable detection secondary antibody and optimize antibody dilution.
• This antibody cross-reacts with mouse, allowing its use in murine models for DNA damage studies.

CamelBio: Your One-Stop Sourcing Bridge

As a reliable IVD raw material partner, CamelBio provides high-quality monoclonal antibodies like anti-53BP1 for DNA damage repair research and diagnostic development. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and specialized sourcing of rare-target raw materials to accelerate your assay development from concept to clinic.