Anti-53BP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q12888

Core Product Specifications and Parameters

Biological Background: TP53-binding protein 1 Function and Localization

• 53BP1, also known as p202, TDRD30, or p53BP1, is a key double-strand break (DSB) repair protein involved in DNA damage response, telomere dynamics, and class-switch recombination (CSR) during antibody genesis. Related references: PMID:12364621 PMID:17190600 PMID:21144835

• It promotes non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracts BRCA1-dependent homologous recombination (HR) repair. Related references: PMID:22553214 PMID:23333306 PMID:23727112

• Upon DNA damage, ATM-mediated phosphorylation of 53BP1 promotes its interaction with RIF1 and dissociation from TIRR, facilitating recruitment to DSB sites. Related references: PMID:28241136

• 53BP1 is recruited to DSBs by recognizing histone H2A monoubiquitinated at K15 (H2AK15Ub) and histone H4 dimethylated at K20 (H4K20me2). Related references: PMID:17190600 PMID:23760478 PMID:27153538

• It is required for immunoglobulin class-switch recombination (CSR), a process that generates DNA DSBs. Related references: PMID:23345425

• 53BP1 also promotes NHEJ at dysfunctional telomeres through interaction with PAXIP1. Related references: PMID:23727112

• Subcellularly, 53BP1 localizes to the nucleus, chromosomes, and specifically to centromeres and kinetochores.

• Alternative splicing produces multiple 53BP1 isoforms, contributing to functional diversity in DNA repair.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a recombinant fragment (aa 895-1031), it is expected to recognize the full-length 53BP1 protein (~214 kDa) and possibly degradation products in western blotting. Optimize exposure and loading controls.
• For WB, a typical starting dilution range of 1:500–1:2000 is often used for polyclonal antibodies; however, the optimal working concentration must be determined empirically in your sample system.
• In IHC-P, antigen retrieval may be required due to the nuclear localization of 53BP1; validate staining specificity with appropriate tissue controls and consider using a nuclear counterstain.
• The antibody shows cross-reactivity with mouse and rat 53BP1, as confirmed by the supplier, making it useful for translational studies; however, sequence alignment of the immunogen region across species should be considered.
• Since 53BP1 is a phosphoprotein involved in DNA damage signaling, treatment of cells with DNA-damaging agents (e.g., ionizing radiation, etoposide) may enhance detection of foci formation in immunofluorescence or modulate signal in WB.
• For ELISA, the antibody can be used to capture or detect recombinant 53BP1 fragments or cell lysates; appropriate blocking and washing conditions should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering validated antibody pairs, bulk ancillary reagents, and rare target raw materials. This anti-53BP1 polyclonal antibody supports research into DNA damage repair pathways and chromatin biology, two areas where CamelBio’s portfolio can accelerate assay development. Reach out to explore integrated supply solutions that span from target discovery to clinical diagnostics.