Anti-4F2hc/CD98/SLC3A2 Monoclonal Antibody for WB, ELISA - P10852

Core Product Specifications and Parameters

Biological Background: 4F2hc/CD98/SLC3A2 Function and Localization

• SLC3A2 acts as a chaperone for SLC7 family light chains, forming heterodimeric amino acid antiporters essential for their plasma membrane trafficking and function (PubMed:10574970, PubMed:10734121, PubMed:11011012). Related references: PMID:10574970, PMID:10734121, PMID:11011012
• The heterodimeric combinations determine substrate specificity, mediating the exchange of neutral, dibasic, and anionic amino acids, as well as thyroid hormones and L-DOPA.
• When SLC3A2/SLC7A5 associates with LAPTM4B, the complex is directed to lysosomal membranes to import leucine, thereby activating mTORC1 signaling.
• SLC3A2 modulates integrin-related signaling and is critical for integrin-dependent cell spreading, migration, and tumor progression.
• The protein localizes to multiple membrane compartments, including apical and basolateral plasma membrane, cell junctions, lysosomes, and melanosomes.
• Broadly expressed across tissues, with highest levels in kidney, small intestine, spleen, thymus, and liver; also detected on brain capillary endothelia (PubMed:9915839). Related references: PMID:9915839
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination; the structure is stabilized by disulfide bonds and a signal-anchor transmembrane segment.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a band near 58 kDa; note that glycosylation may increase the apparent molecular weight.
• In ELISA, recombinant protein corresponding to amino acids 105–526 can be employed as a coating antigen or calibrator to improve consistency.
• If applying the antibody to immunoprecipitation or immunofluorescence, perform titration and validation in the relevant sample system, as the antibody is formally validated only for WB and ELISA.
• Given cross-reactivity with rat, confirm reagent performance in rat tissues or cell lysates using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, from monoclonal antibodies and matched pairs to bulk ancillary reagents, supporting your transition from concept to clinic. This anti-4F2hc/CD98/SLC3A2 product addresses research into amino acid transport and integrin-mediated cellular processes, areas where our supply chain ensures reliable access to both common and rare-target materials. Let us streamline your sourcing with tailored solutions and expert technical consultation.