Anti-4-1BBL/CD137L Rabbit Monoclonal Antibody for WB, ELISA - P41273

Core Product Specifications and Parameters

Biological Background: 4-1BBL/CD137L Function and Localization

• 4-1BBL (CD137L) is a member of the tumor necrosis factor (TNF) ligand superfamily, encoded by the TNFSF9 gene. It is also known as TNLG5A.
• It functions as a cytokine that binds to its receptor TNFRSF9 (4-1BB), delivering co-stimulatory signals that induce proliferation of activated peripheral blood T-cells.
• May play a role in activation-induced cell death (AICD) and in cognate interactions between T-cells and B-cells or macrophages.
• Subcellular localization is primarily at the plasma membrane (single-pass type II membrane protein), consistent with its role as a cell-surface ligand.
• Tissue expression is detected in brain, placenta, lung, skeletal muscle, and kidney.
• Keywords include 3D-structure, cytokine, membrane, and reference proteome, highlighting its structural characterization and biological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 155–254 of human 4-1BBL, mapping to the extracellular domain. This region is highly conserved between human, mouse, and rat, explaining the observed cross-reactivity.
• For Western blot (WB), the expected band size is approximately 27 kDa (unglycosylated). Post-translational modifications or protein context may cause slight mobility shifts.
• ELISA performance may depend on the specific assay format; consider using the antibody in a sandwich ELISA paired with another 4-1BBL antibody recognizing a different epitope.
• Since 4-1BBL is membrane-bound and also exists in soluble form, cell lysate validation in relevant cell lines (e.g., activated T-cells) is recommended to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a reliable one-stop solution for IVD raw materials. This 4-1BBL rabbit monoclonal antibody is suitable for immuno-oncology and autoimmune disease research, complementing our portfolio of immune checkpoint antibodies and recombinant proteins. We also support custom antibody development, bulk reagent supply, and rare target sourcing to accelerate your assay development from concept to clinic.