Anti-4-1BB/CD137 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q07011

Core Product Specifications and Parameters

Biological Background: 4-1BB/CD137 Function and Localization

• 4-1BB (CD137, ILA), encoded by TNFRSF9, is a member of the tumor necrosis factor receptor superfamily and serves as a receptor for the ligand TNFSF9/4-1BBL (Probable).
• Upon ligand binding, 4-1BB conveys costimulatory signals that enhance CD8+ T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
• The receptor is primarily localized on the cell membrane, consistent with its role as a transmembrane signaling receptor.
• Its expression is mainly restricted to the surface of activated T-cells, underlying its role in adaptive immune responses.
• Structurally, 4-1BB contains a signal peptide, a characteristic cysteine-rich extracellular domain with disulfide bonds, and a transmembrane helix; it is also glycosylated.
• Alternative names include 4-1BB ligand receptor, CDw137, T-cell antigen ILA, reflecting historical designations in immunology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 100–200, located within the extracellular domain of human 4-1BB; this region is accessible for antibody binding in native membrane-bound receptor.
• For Western blot, the predicted molecular weight of human 4-1BB is approximately 28 kDa; however, observed bands may shift due to glycosylation and/or dimerization.
• In IHC-P applications, consider standard antigen retrieval techniques and validate staining in human, mouse, or rat tissues known to contain activated T-cells.
• For ELISA, a recombinant 4-1BB protein or a peptide encompassing the immunogen sequence can serve as a positive control; assay conditions may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials and technical services. This 4-1BB/CD137 polyclonal antibody complements our portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunology research. Whether you need rare target sourcing or scalable raw-material supply, CamelBio bridges the gap from concept to clinic.