Anti-15-PGDH/HPGD Polyclonal Antibody for WB, ELISA - P15428

Core Product Specifications and Parameters

Biological Background: 15-PGDH/HPGD Function and Localization

• 15-PGDH (HPGD/SDR36C1) is a member of the short-chain dehydrogenase/reductase family that catalyzes NAD-dependent oxidation of hydroxylated polyunsaturated fatty acids, including prostaglandins, lipoxins, and resolvins, producing their corresponding keto metabolites. Related references: PMID:10837478, PMID:16757471, PMID:16828555
• By degrading pro-proliferative prostaglandin E2, it acts as a tumor suppressor; its oxo-fatty acid products can abrogate pro-inflammatory cytokine expression. Related references: PMID:15574495, PMID:25586183
• It converts resolvins E1, D1, and D2 to oxo metabolites, thereby inactivating these resolution-phase mediators, which impacts acute inflammation and obesity-induced adipose inflammation. Related references: PMID:16757471, PMID:22844113
• Subcellularly localized to the cytoplasm.
• Detected at the protein level in colon epithelium.
• Keywords: Alternative splicing, Disease variant, Fatty acid metabolism, Lipid metabolism, NAD, Oxidoreductase, Prostaglandin metabolism, Tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human 15-PGDH (amino acids 1-266), suggesting recognition of multiple epitopes; perform appropriate validation in the relevant sample system.
• For Western blotting, a band of approximately 29 kDa is expected; include positive and negative controls and consider using reducing conditions.
• In ELISA applications, optimize coating antigen and detection conditions; this polyclonal antibody may be suitable for capture or detection formats depending on assay design.
• Cross-reactivity with mouse and rat has been observed; verify performance and specificity in these species using appropriate tissue or cell lysates.

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