Anti-14-3-3 zeta/YWHAZ Monoclonal Antibody for WB, IF/ICC, ELISA - P63104

Core Product Specifications and Parameters

Biological Background: 14-3-3 zeta/YWHAZ Function and Localization

• 14-3-3 zeta/delta (also known as KCIP-1) is an adapter protein that regulates a wide range of general and specialized signaling pathways. Related references: PMID:14578935 | PMID:15071501 | PMID:15644438
• It binds to numerous protein partners primarily via recognition of phosphoserine or phosphothreonine motifs, thereby modulating their activity. Related references: PMID:35662396
• The protein promotes cytosolic retention and inactivation of TFEB transcription factor by binding phosphorylated TFEB. Related references: PMID:35662396
• It induces ARHGEF7 activity on RAC1, leading to lamellipodia and membrane ruffle formation. Related references: PMID:16959763
• In neurons, 14-3-3 zeta/delta regulates spine maturation through modulation of ARHGEF7 activity (By similarity).
• Subcellular localization includes the cytoplasm and melanosomes, and the protein undergoes post-translational modifications such as phosphorylation and acetylation. It is associated with disease variants in intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 145-245 of human YWHAZ, suggesting the epitope lies within this region. Consider this when designing peptide competition or epitope mapping experiments.
• For Western blot (WB), expect a primary band around 28 kDa (observed; theoretical molecular weight is 27.7 kDa). Use appropriate molecular weight markers and loading controls.
• For immunofluorescence (IF/ICC) and ELISA, validate the antibody in your relevant sample system, as performance may vary depending on fixation and permeabilization conditions.
• The antibody cross-reacts with human, mouse, and rat, making it suitable for multi-species studies. Confirm species-specific reactivity if your experiment involves closely related proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated procurement partner for IVD raw materials, offering this high-quality anti-14-3-3 zeta/YWHAZ monoclonal antibody as part of a comprehensive portfolio that includes validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are developing diagnostic assays for signaling pathway research or other applications, contact us to streamline your supply chain from concept to clinic.