Anti-14-3-3 Theta Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P27348

Core Product Specifications and Parameters

Biological Background: 14-3-3 Theta Function and Localization

• 14-3-3 protein theta (YWHAQ) is a member of the 14-3-3 family of adapter proteins, also known as 14-3-3 protein T-cell or protein HS1.
• It functions as a versatile adapter that regulates a broad spectrum of signaling pathways by binding to phosphoserine or phosphothreonine motifs on partner proteins.
• One key interaction includes negative regulation of the kinase activity of PDPK1, influencing downstream signaling cascades.
• The protein is predominantly localized to the cytoplasm and is abundantly expressed in the brain, heart, and pancreas, with lower levels in kidney and placenta.
• It undergoes various post-translational modifications, including phosphorylation, acetylation, nitration, and ubiquitin-like conjugation, which may modulate its function and interactions.
• Notably, 14-3-3 theta expression is upregulated in the lumbar spinal cord of patients with sporadic amyotrophic lateral sclerosis (ALS), particularly in cases with predominant lower motor neuron involvement, suggesting a role in neurodegeneration.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band near 28 kDa; consider using a high-quality blocking buffer and optimizing primary antibody concentration.
• In immunofluorescence/ICC, anticipate cytoplasmic staining; validate fixation and permeabilization conditions for your cell type to preserve epitope integrity.
• The peptide immunogen (aa 1-100) may support ELISA applications; verify linear range and specificity with appropriate antigen controls in your assay system.
• Cross-reactivity with mouse and rat allows translational studies, but always confirm reactivity in your experimental model.

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CamelBio supports diagnostic manufacturers and researchers with reliable access to IVD raw materials, including validated monoclonal antibodies like this anti-14-3-3 theta clone. Our integrated supply capabilities extend to antibody pairs, ancillary reagents, and rare-target sourcing, helping streamline your development workflow from early research through clinical application. Reach out to explore bulk options or customized solutions tailored to your neuroscience and cell signaling projects.