Anti-14-3-3 sigma Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P31947

Core Product Specifications and Parameters

Biological Background: 14-3-3 sigma Function and Localization

• Acts as an adapter protein that binds a wide range of partners primarily through recognition of phosphoserine or phosphothreonine motifs, modulating their activity in diverse signaling pathways.
  Related references: PMID:15731107, PMID:22634725, PMID:28202711
• Functions as a TP53/p53-regulated inhibitor of G2/M progression, thereby contributing to cell cycle checkpoint control.
  Related references: PMID:9659898
• Involved in innate immune regulation by promoting cytosolic retention of phosphorylated GBP1 GTPase, which inhibits the innate immune response.
  Related references: PMID:37797010
• Maintains desmosome cell junction adhesion in epithelial cells by sequestering PKP3 in the cytoplasm, regulating its translocation and preserving desmosome protein homeostasis.
  Related references: PMID:24124604, PMID:29678907
• May regulate MDM2 autoubiquitination and degradation, thereby activating p53/TP53.
  Related references: PMID:18382127
• Predominantly expressed in tissues enriched in stratified squamous keratinizing epithelium, highlighting its role as an epithelial cell marker.
• Localizes to the cytoplasm, nucleus, and is also secreted; classified as a phosphoprotein and is subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N‑terminal region (aa 1‑100); consider that the antibody may preferentially recognize denatured protein in WB.
• For IHC‑P, validate antigen retrieval methods and antibody dilution in relevant epithelial tissues.
• In ELISA applications, test pairing with other 14‑3‑3 sigma antibodies and optimize coating and detection conditions.
• Confirm specificity via knockdown or knockout controls in your cell or tissue system of interest.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This 14‑3‑3 sigma antibody supports research and assay development in oncology, cell signaling, and epithelial biology. Beyond single antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—helping you bridge raw material procurement to final product with ease.