Anti-14-3-3 sigma Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P31947

Core Product Specifications and Parameters

Biological Background: 14-3-3 sigma Function and Localization

• 14-3-3 sigma (also known as Stratifin or Epithelial cell marker protein 1) is an adapter protein encoded by the SFN gene, predominantly expressed in tissues enriched in stratified squamous keratinizing epithelium.
• It functions by binding phosphoserine/phosphothreonine motifs on a broad range of signaling partners, generally modulating their activity, and is implicated in pathways such as cell cycle, apoptosis, and growth signaling (PubMed:15731107, PubMed:22634725, PubMed:28202711). Related references: PMID:15731107, PMID:22634725, PMID:28202711
• It acts as a TP53/p53-regulated inhibitor of G2/M cell cycle progression, contributing to the DNA damage checkpoint (PubMed:9659898). Related references: PMID:9659898
• 14-3-3 sigma promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Related references: PMID:37797010
• In epithelial cells, it maintains desmosome cell junction adhesion by interacting with and sequestering PKP3 to the cytoplasm, which facilitates exchange at desmosome plaques and preserves intercellular adhesion (PubMed:24124604, PubMed:29678907). Related references: PMID:24124604, PMID:29678907
• The protein may regulate MDM2 autoubiquitination and degradation, thereby activating p53/TP53 (PubMed:18382127). Related references: PMID:18382127
• Subcellular localization includes the cytoplasm, nucleus, and secretion, consistent with its diverse roles in signal transduction and intercellular communication.
• Key post-translational modifications include phosphorylation and ubiquitin conjugation, which fine-tune its functions and interactions.
• Tissue expression is largely restricted to stratified squamous epithelia, making it a useful marker for epithelial differentiation and pathology studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (amino acids 1-100) of human 14-3-3 sigma. This region may contain critical phosphoepitopes; ensure your sample preparation preserves phosphorylation states if relevant.
• For Western blot (WB), anticipate a band around 28 kDa (calculated molecular weight is ~27.8 kDa). Validate with appropriate positive controls, such as lysates from stratified epithelial tissue or cell lines (e.g., HaCaT, A431) where endogenous expression is expected.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods (e.g., citrate buffer, pH 6.0) and verify specificity using skin, esophagus, or other squamous epithelium as positive control tissues.
• In ELISA, the peptide-based immunogen allows direct coating of the peptide for standard assay setups; titrate antibody concentration to optimize signal-to-noise.
• Cross-reactivity with mouse 14-3-3 sigma is reported; however, sequence alignment across species is recommended before applying to other model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom procurement of rare targets. To support your 14-3-3 sigma research in cancer biology, cell signaling, and epithelial differentiation, CamelBio provides reliable access to this and other related products, streamlining your path from discovery to assay development.