Anti-14-3-3 gamma Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P61981

Core Product Specifications and Parameters

Biological Background: 14-3-3 gamma Function and Localization

• Encoded by gene YWHAG, 14-3-3 protein gamma is a member of the 14-3-3 family of highly conserved adapter proteins.
• Functions as a scaffold and adapter that binds phosphoserine/threonine motifs on diverse partners, modulating numerous signaling pathways. Related references: PMID:15696159, PMID:16511572, PMID:36732624
• Negatively regulates mTORC1 signaling by binding to phosphorylated WDR24 and promoting inactivation of the GATOR2 complex. Related references: PMID:36732624
• Enhances NMDA glutamate receptor activity through L-glutamate secretion via interaction with BEST1. Related references: PMID:29121962
• Localizes predominantly to the cytoplasm and cytosol, and is also found in the mitochondrial matrix where it participates in mitochondrial protein degradation (MALM). Related references: PMID:22532927
• Highly expressed in brain, skeletal muscle, and heart, aligning with its roles in neurological and muscle functions.
• Post-translationally modified by phosphorylation and acetylation; mutations are linked to developmental and epileptic encephalopathy.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to human 14-3-3 gamma (aa 100-200); consider sequence alignment to verify epitope conservation if using non-human samples.
• For Western blot, the expected band size is approximately 28 kDa; include appropriate positive controls (e.g., brain, skeletal muscle lysates) where 14-3-3 gamma is highly expressed.
• For IHC-P, pilot testing with antigen retrieval conditions is recommended; use cross-reactive tissues as validated for human, mouse, and rat.
• In ELISA, pair with a suitable detection antibody; confirm working concentration via checkerboard titration.

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