Anti-14-3-3 epsilon Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62258

Core Product Specifications and Parameters

Biological Background: 14-3-3 epsilon Function and Localization

• 14-3-3 epsilon (gene YWHAE) is a 29 kDa adapter protein belonging to the 14-3-3 family, which recognizes phosphoserine/threonine motifs to modulate partner protein activity. Related references: PMID:35343654
• Functions as a central signaling regulator, binding numerous partners to influence pathways such as cell cycle control, apoptosis, and innate immunity (PubMed:21189250). Related references: PMID:21189250
• Positively regulates the antiviral response by directing RIGI redistribution to mitochondrial membranes, facilitating MAVS-dependent signaling (PubMed:22607805, PubMed:37555661). Related references: PMID:22607805 PMID:37555661
• Mediates nuclear export of phosphorylated HSF1, a key step in heat shock response attenuation (PubMed:12917326). Related references: PMID:12917326
• Promotes cell proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus for ubiquitination-dependent degradation (PubMed:37599448). Related references: PMID:37599448
• Localizes to the nucleus, cytoplasm, and melanosomes, reflecting its dynamic shuttling and diverse functional contexts.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), which regulate its interactions and stability.

Experimental Guidance and Technical Tips

• The immunogen (aa 73‑163) targets a conserved central region, suggesting suitability for detecting human, mouse, and rat 14-3-3 epsilon in Western blotting. For optimal band detection at ~29 kDa, consider using a 12% SDS‑PAGE gel and appropriate membrane transfer conditions.
• For immunofluorescence/ICC, include permeabilization steps and validate fixation conditions, as 14-3-3 epsilon shuttles between the nucleus and cytoplasm; non‑specific staining may be reduced by titrating the antibody.
• ELISA performance may require optimizing coating conditions due to the polyclonal nature; test with recombinant human 14-3-3 epsilon as a positive control and include proper blocking.

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