Anti-14-3-3 epsilon Monoclonal Antibody for WB, ELISA - P62258

Core Product Specifications and Parameters

Biological Background: 14-3-3 epsilon Function and Localization

• 14-3-3 epsilon is an adapter protein implicated in regulating a broad spectrum of signaling pathways by binding phosphoserine/threonine motifs on partner proteins, thereby modulating their activity.
  Related references: PMID:21189250, PMID:35343654
• It positively regulates the antiviral innate immune response by interacting with RIGI, directing its redistribution to mitochondrial‑associated membranes for MAVS‑dependent signaling, and functions upstream of TBK1.
  Related references: PMID:37555661, PMID:22607805
• 14-3-3 epsilon plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm for ubiquitination‑dependent degradation.
  Related references: PMID:37599448
• It positively regulates the nuclear export of phosphorylated HSF1 to the cytoplasm.
  Related references: PMID:12917326
• Subcellular localization is predominantly cytoplasmic and nuclear, and the protein has also been detected in melanosomes.
• Post‑translational modifications include acetylation, phosphorylation, and ubiquitination (Ubl conjugation), and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide within the N‑terminal region (amino acids 1–100) of human 14-3-3 epsilon. Based on sequence homology, cross‑reactivity with mouse and rat is expected; however, validation in the relevant sample system is recommended.
• Suitable for Western blot (WB) and ELISA applications. For WB, consider using whole‑cell lysates from human, mouse, or rat cell lines, as the predicted molecular weight is approximately 28 kDa.
• Optimal working dilutions and conditions should be determined experimentally by the end‑user; no specific dilutions are implied.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For 14-3-3 epsilon, a key adapter protein in cell signaling and innate immunity, we support diagnostic developers with reliable raw materials and customized sourcing solutions to accelerate assay development from concept to clinic.