Anti-14-3-3 beta/zeta Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P31946 / P63104

Core Product Specifications and Parameters

Biological Background: 14-3-3 beta/zeta Function and Localization

• 14-3-3 beta/alpha (gene YWHAB) is an adapter protein that regulates a broad range of signaling pathways by binding to phosphoserine or phosphothreonine motifs on partner proteins.
• It functions as a negative regulator of osteogenesis, inhibiting bone formation.
• Blocks the AKT1-mediated nuclear translocation of SRPK2, thereby antagonizing SRPK2-induced cyclin D1 expression and preventing neuronal apoptosis.
• Acts as a negative regulator of MAP kinase signaling cascades through interactions with AKAP13.
• Subcellularly localizes to the cytoplasm, melanosomes, and vacuole membranes, indicating roles in diverse cellular compartments.
• Keywords include phosphoprotein, acetylation, and Ubl conjugation, suggesting regulation by multiple post-translational modifications.
• The highly conserved 14-3-3 family includes beta and zeta isoforms, which share extensive functional overlap and sequence homology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human 14-3-3 beta (P31946), a domain highly conserved among 14-3-3 family members, including zeta. This may contribute to the dual specificity.
• For Western blot (WB), expect a band around 28 kDa for 14-3-3 beta/zeta. Consider validating in lysates from human, mouse, or rat tissues/cells as indicated by the cross-reactivity data.
• In immunofluorescence (IF/ICC), anticipate cytoplasmic and possibly membrane-associated staining patterns consistent with the reported subcellular localization.
• ELISA may be useful for quantifying binding interactions, but optimal coating and detection conditions should be empirically determined.
• These applications are supported by the supplier’s specifications; individual assay performance should be verified with appropriate controls.

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CamelBio offers comprehensive access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-14-3-3 beta/zeta clone, ideal for studies in signal transduction, cell cycle regulation, and neurobiology. As a dedicated partner, we streamline your procurement of antibody pairs, bulk ancillary reagents, and rare target raw materials, supporting your diagnostic and research workflows from concept to clinic.