Anti-14-3-3-beta/zeta Rabbit Monoclonal Antibody for WB, ELISA - P31946 / P63104

Core Product Specifications and Parameters

Biological Background: 14-3-3 beta/zeta Function and Localization

• The 14-3-3 protein beta/alpha (gene YWHAB) and zeta/delta (gene YWHAZ) are highly conserved adapter proteins that regulate diverse signaling pathways by binding phosphorylated serine/threonine motifs.
• 14-3-3 proteins form homo- and heterodimers and interact with numerous partners, modulating their activity, localization, and stability.
• 14-3-3 beta/alpha acts as a negative regulator of osteogenesis and can block apoptosis by preventing nuclear translocation of phosphorylated SRPK2, thereby inhibiting cyclin D1 expression.
• It negatively regulates MAP kinase signaling cascades through interaction with AKAP13.
• Subcellular localization includes the cytoplasm, melanosomes, and vacuole membranes, indicating roles in intracellular trafficking and organelle function.
• Keywords highlight post-translational modifications such as acetylation, phosphorylation, nitration, and ubiquitination, which may regulate its activity and interactions.
• It is implicated in host-virus interactions and has been identified through proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of 14-3-3 beta, which shares high homology with zeta. This region is likely exposed and suitable for detection of both isoforms in Western blot and ELISA.
• For WB, a band around 28 kDa is expected. Validate the antibody in relevant sample systems (human, mouse, rat) as cross-reactivity is reported.
• Consider optimizing antibody dilution and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined sourcing for IVD raw materials worldwide, supporting diagnostic manufacturers and laboratories from concept to clinic. This anti-14-3-3 beta/zeta rabbit monoclonal antibody is ideal for research into signaling pathways, apoptosis, and oncology. We provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.