Anti-14-3-3 alpha/beta Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P31946

Core Product Specifications and Parameters

Biological Background: 14-3-3 beta/alpha Function and Localization

• 14-3-3 protein beta/alpha (also known as Protein 1054 or Protein kinase C inhibitor protein 1, KCIP-1) is encoded by the YWHAB gene in humans. It belongs to the 14-3-3 family of conserved regulatory proteins.
• Functions as an adapter protein that binds to a large number of signaling partners, typically via recognition of phosphoserine or phosphothreonine motifs, modulating their activity.
• Acts as a negative regulator of osteogenesis and blocks neuronal apoptosis by inhibiting the AKT1-phosphorylated SRPK2-mediated upregulation of cyclin D1.
• Negatively regulates MAP kinase signaling cascades mediated by AKAP13.
• Localizes to the cytoplasm, melanosomes, and vacuole membrane.
• Subject to multiple post-translational modifications including acetylation, nitration, phosphorylation, and ubiquitination (isopeptide bond formation).
• Involved in host-virus interactions and has been identified through direct protein sequencing and proteomics.
• The protein has a molecular weight of approximately 28 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 150–246, suggesting the antibody recognizes the C-terminal region of human 14-3-3 beta/alpha. Sequence alignment with mouse and rat orthologs indicates potential cross-reactivity, consistent with the supplier-reported cross-reactivity.
• For Western blot (WB), a band around 28 kDa is expected. Consider using appropriate positive control lysates (e.g., HeLa, HEK293) and validate specificity with siRNA or blocking peptide.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution should be empirically optimized in the relevant tissue type.
• This polyclonal antibody may be suitable for immunoprecipitation (IP) and ELISA; for ELISA, the synthetic peptide immunogen could serve as a positive control.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified in critical assays, and appropriate secondary antibodies should be selected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs and bulk reagents. For 14-3-3 signaling research, we offer a range of anti-14-3-3 antibodies and ancillary proteins to support assay development. Our sourcing capabilities extend from routine supplies to rare target reagents, helping you transition from concept to clinic efficiently.