Anti-14-3-3 alpha/beta Monoclonal Antibody for WB, ELISA - P31946

Core Product Specifications and Parameters

Biological Background: 14-3-3 alpha/beta Function and Localization

• 14-3-3 beta/alpha (gene YWHAB) is a member of the 14-3-3 protein family, highly conserved adapter proteins that bind phosphoserine/threonine motifs and regulate diverse signaling pathways.
• It functions as an adapter protein implicated in a wide spectrum of signaling cascades, including those controlling cell cycle progression, apoptosis, and metabolism.
• Acts as a negative regulator of osteogenesis and MAP kinase signaling via AKAP13.
• Blocks AKT1-mediated nuclear translocation of SRPK2, antagonizing cyclin D1 expression and preventing neuronal apoptosis.
• Localizes predominantly to the cytoplasm, and is also detected in melanosomes and vacuole membranes.
• Post-translational modifications include phosphorylation, acetylation, nitration, and ubiquitination, which modulate its binding specificity and functional outcomes.
• Involved in host-virus interactions, indicating a role in viral pathogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 147-246 of human 14-3-3 alpha/beta; consider that the antibody may recognize an area involved in phosphopeptide binding, and test phosphorylation-dependent reactivity if relevant.
• Recommended applications are WB and ELISA; for staining, validate the antibody in your specific sample system and include appropriate positive/negative controls.
• Given high sequence conservation, cross-reactivity with mouse and rat is expected; verify with species-specific controls to confirm specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. This 14-3-3 alpha/beta monoclonal antibody supports investigation into signaling pathways, apoptosis, and osteogenesis. Count on us for seamless procurement and expert support from concept to clinic.