Anti-12 Lipoxygenase/ALOX12 Monoclonal Antibody for WB, IF-P, ELISA - P18054

Core Product Specifications and Parameters

Biological Background: 12 Lipoxygenase/ALOX12 Function and Localization

• ALOX12 is a polyunsaturated fatty acid lipoxygenase, also known as 12S-lipoxygenase or platelet-type lipoxygenase, encoded by the ALOX12 gene (Gene ID: 239).
• It catalyzes the regio- and stereo-specific oxygenation of free and esterified polyunsaturated fatty acids to generate lipid hydroperoxides, with arachidonate as a major substrate yielding (12S)-HPETE. Related references: PMID:17493578, PMID:18311922, PMID:1851637
• Through (12S)-HPETE production, it regulates platelet activation. Related references: PMID:8319693, PMID:8500694
• It also catalyzes the epoxidation of double bonds, e.g., converting (14S)-HPDHA to (13S,14S)-epoxy-DHA, and participates in the biosynthesis of specialized pro-resolving mediators like resolvin D5. Related references: PMID:23504711, PMID:32404334
• ALOX12 transforms leukotriene A4 into the vasoactive lipoxins LXA4 and LXB4, modulating neutrophil function. Related references: PMID:8250832
• It is considered protumorigenic, promoting VEGF expression and integrin beta-1 to influence tumor survival and metastasis. Related references: PMID:16638750, PMID:22237009, PMID:9751607
• ALOX12 also plays a role in preventing apoptosis in vascular smooth muscle cells. Related references: PMID:23578768
• Subcellular localization includes cytoplasm, cytosol, and membrane; tissue expression is noted in vascular smooth muscle cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (aa 483‑663) of human ALOX12; consider sequence alignment to verify epitope conservation in cross‑reactive species (mouse, rat).
• For Western blot, a band at approximately 76 kDa is expected; validate with appropriate positive controls such as human platelet lysates or vascular smooth muscle cells.
• In immunofluorescence (IF‑P), fixation and permeabilization conditions may influence detection; optimize for your cell type and consider inclusion of organelle markers to assess localization.
• For ELISA, the antibody may be used for capture or detection; pair with appropriate standards and perform cross‑reactivity testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one‑stop IVD raw‑material supplier providing validated monoclonal antibodies like this Anti‑ALOX12 clone to diagnostic manufacturers and researchers worldwide. We offer comprehensive solutions including optimized antibody pairs, bulk ancillary reagents, and customized sourcing for lipid signaling and platelet biology targets. Partner with us to accelerate your assay development from concept to clinic.