ANPRB/NRP2 Rabbit pAb - P20594

Core Product Specifications and Parameters

Biological Background: ANPRB/NRP2 Function and Localization

• ANPRB/NRP2, also known as atrial natriuretic peptide receptor type B or guanylate cyclase B (GC-B), is encoded by the NPR2 gene (GeneID: 4882). It functions as a receptor for C-type natriuretic peptide (CNP/NPPC).
• Upon CNP binding, the receptor's intracellular guanylate cyclase domain catalyzes the synthesis of cyclic GMP (cGMP), an important second messenger in signal transduction.
• The protein is a single-pass transmembrane receptor located at the cell membrane, forming homodimers stabilized by disulfide bonds.
• ANPRB/NRP2 plays a critical role in endochondral ossification and skeletal growth; loss-of-function mutations lead to dwarfism (acromesomelic dysplasia, Maroteaux type) and other skeletal dysplasias.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which modulate receptor activity and downstream signaling.
• Alternative splicing generates multiple isoforms, though the canonical full-length protein (precursor MW ~117 kDa) is the major functional form.
• UniProt keywords highlight: Alternative splicing, Cell membrane, cGMP biosynthesis, Disease variant, Disulfide bond, Dwarfism, Glycoprotein, GTP-binding, Lyase, Membrane, Nucleotide-binding, Osteogenesis, Phosphoprotein, Receptor, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of ANPRB/NRP2 is approximately 117 kDa. Use a suitable human tissue or cell line lysate as positive control (e.g., osteoblasts, chondrocytes, or vascular smooth muscle cells) and validate band specificity with appropriate controls.
• For ELISA applications, this polyclonal antibody can be employed as a capture or detection reagent; however, optimal coating conditions and antibody titrations should be experimentally determined.
• The immunogen (aa 130–320) lies within the extracellular domain, which is involved in ligand interaction. Confirm that the antibody recognizes both denatured (WB) and native (ELISA) protein conformations if required for your assay.
• Given the receptor's role in skeletal growth and cGMP signaling, consider testing in relevant model systems (e.g., differentiating primary cells or induced pluripotent stem cell-derived chondrocytes) to assess functional blocking potential if needed.
• Always validate antibody specificity in your specific experimental context using knockdown, overexpression, or blocking peptide controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For projects targeting the CNP/NPR2 signaling pathway or skeletal growth regulation, we provide a robust portfolio including validated polyclonal antibodies like this ANPRB/NRP2 pAb, optimized antibody pairs, and bulk ancillary reagents. Our expertise extends to sourcing rare and specialized raw materials, empowering your assay development with reliable, high-quality components.