Annexin A6/Annexin VI Rabbit Polyclonal Antibody for WB, ELISA - P08133

Core Product Specifications and Parameters

Biological Background: Annexin A6/Annexin VI Function and Localization

• Annexin A6, also known as Annexin VI, is a member of the annexin family of calcium-dependent phospholipid-binding proteins, characterized by a core domain composed of multiple annexin repeats.
• The protein is alternatively spliced, producing isoforms with molecular weights around 68 kDa (p68) and 70 kDa (p70), and exhibits post-translational modifications including acetylation and phosphorylation.
• Annexin A6 may associate with CD21 and is implicated in the regulation of intracellular calcium release, contributing to calcium signaling homeostasis.
• Subcellular localization includes the cytoplasm and melanosomes, reflecting roles in membrane trafficking and organelle function.
• The human ANXA6 gene is located on chromosome 5q33.1 and encodes a protein of 673 amino acids (isoform 1), with a calculated molecular mass of approximately 75.9 kDa.
• Structural studies have revealed a flexible linker connecting the two annexin repeat halves, enabling the protein to bridge membranes and scaffold protein complexes.

Experimental Guidance and Technical Tips

• For western blotting, the immunogen corresponds to the N-terminal region (aa 1-250), suggesting detection of both predominant isoforms around 76 kDa; consider using appropriate molecular weight markers and tissue/cell lysates.
• As a polyclonal antibody, lot-to-lot consistency may vary; validate performance in your specific sample system and consider pre-adsorption if non-specific bands appear.
• In ELISA, this antibody may be employed for antigen capture or detection; pairing with a different clone or tagged recombinant protein can enhance assay specificity.
• Cross-reactivity with mouse and rat indicates conserved epitopes; however, confirm reactivity levels in your target species and tissue context using a known positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive sourcing of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this Annexin A6 reagent. Our supply network covers bulk ancillary reagents and rare-target raw materials, streamlining your transition from concept to clinic. Whether you need custom production or ready-to-use antibodies for calcium signaling and membrane biology applications, we offer reliable procurement solutions tailored to your project timeline.