Annexin A4/Annexin IV Rabbit Polyclonal Antibody for WB, ELISA - P09525

Core Product Specifications and Parameters

Biological Background: Annexin A4 Function and Localization

• Annexin A4, also known as annexin IV, 35-beta calcimedin, PAP-II, and other aliases, is encoded by the ANXA4 gene and belongs to the annexin family, characterized by conserved repeat domains that mediate calcium-dependent phospholipid binding.
• The protein functions as a calcium/phospholipid-binding protein that promotes membrane fusion and participates in exocytosis, a process essential for secretion and vesicle trafficking.
• Membrane fusion activity is critical for cellular exocytic events, likely involving the zymogen granule membrane where Annexin A4 localizes.
• Subcellularly, Annexin A4 is associated with the zymogen granule membrane, indicating a role in regulated secretion pathways.
• Post-translational modifications of Annexin A4 include acetylation and phosphorylation, as identified by proteomics approaches.
• The ANXA4 gene undergoes alternative splicing and alternative initiation, potentially generating multiple isoforms with distinct functional properties.
• The protein contains repeated annexin domains, a hallmark of the family, which fold into a curved disc shape to bind phospholipids in a calcium-dependent manner.

Experimental Guidance and Technical Tips

• The immunogen used covers amino acids 112-321, a region within the annexin repeat domains of human Annexin A4, which may confer specificity among annexin family members.
• For Western blot (WB), consider a protein molecular weight of 36 kDa for human Annexin A4; validate in your sample system as post-translational modifications or isoform differences could alter migration.
• For ELISA, this polyclonal antibody may offer broad epitope recognition; perform appropriate controls and titrate the antibody for optimal signal-to-noise ratio.
• This antibody recognizes Annexin A4 from human, mouse, and rat; verify cross-reactivity in your species of interest as homology in the immunogen region is high but not absolute.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For Annexin A4-related studies in membrane biology and exocytosis, we can further support your project with technical services and consulting from concept to clinic. Our one-stop model streamlines procurement, enabling efficient development of diagnostic assays.