ANGEL2 Rabbit Polyclonal Antibody for WB, ELISA - Q5VTE6

Core Product Specifications and Parameters

Biological Background: ANGEL2 Function and Localization

• ANGEL2 (also known as Ccr4d or KIAA0759L) is an RNA 2',3'-cyclic phosphatase that specifically hydrolyzes the 2',3'-cyclic phosphate at the 3' end of RNA, generating a linear 3'-phosphate group.
  Related references: PMID:32732418 PMID:36180430

• It plays a critical role in mitochondrial RNA maturation and processing, particularly for non-canonically generated transcripts.

• ANGEL2 antagonizes pre-tRNA processing and XBP1 mRNA splicing during the unfolded protein response by limiting 2',3'-cyclic phosphate substrates for the tRNA ligase complex.
  Related references: PMID:32732418

• The protein promotes tRNA recycling by removing 2',3'-cyclic phosphate groups from cleaved tRNAs, enabling TRNT1 to reattach the CCA tail essential for aminoacylation and translation.
  Related references: PMID:32732418

• Subcellularly, ANGEL2 localizes to the mitochondrial matrix.

• The gene undergoes alternative splicing, and the enzyme requires magnesium as a cofactor; it is classified as a hydrolase involved in both mRNA and tRNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–170 in the N-terminal region of human ANGEL2. Consider verifying antibody recognition of endogenous full-length protein or possible isoforms using recombinant protein controls.
• For Western blot, the predicted molecular weight of ANGEL2 is approximately 62 kDa. Optimize blocking and detection conditions to minimize non-specific binding common with polyclonal preparations.
• In ELISA applications, consider including peptide competition or reference standards to confirm specificity and sensitivity.
• Because ANGEL2 resides in the mitochondrial matrix, ensure appropriate lysis and sample preparation protocols to effectively solubilize this organellar protein from cells or tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ANGEL2 antibody supports mitochondrial RNA biology and stress response research. CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials for your assay development.