ANAPC10 Rabbit pAb - Q9UM13

Core Product Specifications and Parameters

Biological Background: ANAPC10 Function and Localization

• ANAPC10 (APC10/DOC1) is a core subunit of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and G1 phase.
  Related references: PMID:18485873, PMID:27120157, PMID:27509861
• APC/C primarily catalyzes the formation of Lys-11-linked polyubiquitin chains on target proteins, with lesser activity toward Lys-48- and Lys-63-linked chains, and can assemble branched Lys-11/Lys-48-linked ubiquitin chains.
  Related references: PMID:18485873, PMID:29033132
• During mitosis, APC/C is activated by CDC20, leading to the ubiquitination and degradation of cyclin B and securin, thereby triggering the metaphase-to-anaphase transition.
  Related references: PMID:27120157, PMID:27509861
• The mitotic checkpoint complex (MCC) inhibits APC/C activity to delay chromosome segregation until all chromosomes are properly attached to the spindle.
  Related references: PMID:27509861
• ANAPC10 is subject to acetylation, as identified through proteomic analyses, suggesting additional layers of regulation.
• The APC/C and its subunits are part of the ubiquitin conjugation pathway, playing a central role in protein homeostasis and cell cycle checkpoints.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein covering amino acids 1-185 of human ANAPC10, a region likely conserved among human, mouse, and rat, consistent with the observed cross-reactivity.
• This polyclonal antibody is suitable for Western blot (WB), where a band at approximately 21kDa is expected, and for ELISA applications.
• For WB, consider optimizing protein loading amounts, blocking conditions, and primary antibody dilution to achieve clear and specific bands in your sample system.
• The antibody may be used in research on cell cycle regulation, ubiquitination, and mitotic checkpoint signaling; validate performance in your relevant biological context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibodies like this ANAPC10 Rabbit pAb for cell cycle and ubiquitin-related research. Our portfolio includes antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets—covering every stage from concept to clinic.