AMSH Rabbit mAb - O95630

Core Product Specifications and Parameters

Biological Background: AMSH (STAM-binding protein) Function and Localization

• AMSH specifically cleaves Lys-63-linked polyubiquitin chains, acting as a deubiquitinase that regulates receptor endocytosis and lysosomal degradation (PubMed:15314065, PubMed:23542699, PubMed:34425109). Related references: PMID:15314065 PMID:23542699 PMID:34425109
• Unlike its activity toward Lys-63 linkages, AMSH does not cleave Lys-48-linked polyubiquitin chains, demonstrating linkage specificity (PubMed:15314065). Related references: PMID:15314065
• It mediates signal transduction for cell growth and MYC induction via IL-2 and GM-CSF pathways (PubMed:10383417). Related references: PMID:10383417
• AMSH potentiates bone morphogenetic protein (BMP) signaling by counteracting the inhibitory effects of SMAD6 and SMAD7 (PubMed:11483516). Related references: PMID:11483516
• It negatively regulates PI3K-AKT-mTOR and RAS-MAP signaling cascades, implicating it in growth control (PubMed:23542699). Related references: PMID:23542699
• AMSH localizes to multiple subcellular compartments including the nucleus, cytoplasm, membrane, and early endosomes; endosomal localization is essential for efficient EGFR degradation (PubMed:15314065, PubMed:17261583). Related references: PMID:15314065 PMID:17261583
• The protein is ubiquitously expressed and subject to alternative splicing and phosphorylation, with roles in ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• For western blotting, consider using whole-cell lysates from human or monkey cell lines; due to ubiquitous expression, AMSH should be detectable across many tissue types.
• The immunogen peptide corresponds to a central region (aa 100–200); verify specificity with appropriate controls, especially if probing for isoforms.
• For ELISA, titrate the antibody and ensure proper blocking to minimize nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, including validated monoclonal antibodies like this AMSH clone for ubiquitin pathway research. From optimized antibody pairs to bulk ancillary reagents, our one-stop supply model helps accelerate assay development for cell signaling and endocytosis targets. Contact us for reliable sourcing and technical consultation tailored to your IVD project needs.