AMPD3 Rabbit pAb for WB - Q01432

Core Product Specifications and Parameters

Biological Background: AMP Deaminase 3 Function and Localization

• AMP deaminase 3 catalyzes the deamination of adenosine monophosphate (AMP) to inosine monophosphate (IMP), a key step in purine nucleotide metabolism and energy production.
• It is a zinc-binding enzyme and requires metal ions for its catalytic activity.
• Encoded by the AMPD3 gene, it exists as multiple isoforms due to alternative splicing, which may influence tissue-specific regulation.
• Also referred to as erythrocyte AMP deaminase, highlighting its prominent expression in red blood cells.
• As a phosphoprotein, its activity may be modulated by phosphorylation in response to cellular signals.
• Listed under Disease variant in UniProt, implying that genetic variations in AMPD3 could contribute to pathological conditions.
• Belongs to the AMP deaminase family, with roles in maintaining the adenylate energy charge within cells.
• Identified through proteomics, underscoring its presence in various biological samples.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-260 of human AMPD3) covers the N-terminal region; consider this region for epitope mapping or competitive assays.
• For Western Blot, the predicted molecular weight is approximately 89 kDa; use appropriate gel percentage and transfer conditions to resolve this protein.
• Validate antibody performance in relevant sample systems known to express AMPD3, such as erythrocyte lysates.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for orthologous studies; verify species-specific binding in preliminary experiments.
• For ELISA applications, optimize coating antigen concentration and antibody dilution to achieve desired sensitivity and specificity.
• Since AMPD3 undergoes alternative splicing and phosphorylation, ensure the antibody can detect relevant isoforms or modified forms if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing. We support diagnostic manufacturers and research laboratories with efficient, integrated supply solutions. For AMP deaminase 3 and nucleotide metabolism research, explore our portfolio of antibodies and related reagents.