AMPD1 Rabbit pAb for WB, ELISA - P23109

Core Product Specifications and Parameters

Biological Background: AMPD1 Function and Localization

• AMPD1 encodes AMP deaminase 1, also named myoadenylate deaminase or AMP deaminase isoform M.
• The enzyme plays a critical role in energy metabolism by catalyzing the deamination of AMP to IMP in the purine nucleotide cycle.
• It functions in nucleotide metabolism as a hydrolase and requires zinc as a metal cofactor.
• Alternative splicing produces multiple isoforms of the protein.
• The protein is subject to phosphorylation, indicating potential regulation by signaling pathways.
• Mutations in AMPD1 are linked to human disease, including myoadenylate deaminase deficiency (disease variant).
• It has been identified through proteomics studies as a reference proteome component.

Experimental Guidance and Technical Tips

• For western blot (WB), the predicted molecular weight is approximately 86 kDa. Validate the band size using a positive control lysate from human, mouse, or rat tissue, as the antibody cross-reacts with these species.
• Due to alternative splicing, multiple isoforms may be detected; confirm isoform specificity in your experimental system by comparing with known size markers or recombinant proteins.
• For ELISA applications, optimize the coating antigen and antibody dilution to achieve the required sensitivity and specificity. The immunogen peptide may be used as a positive control.
• The immunogen corresponds to the N-terminal region (aa 1–100) of human AMPD1. Assess potential cross-reactivity with other AMPD family members (AMPD2, AMPD3) if your studies require isoform discrimination.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This AMPD1 antibody supports investigations into energy metabolism, nucleotide metabolism, and muscle-related disorders. In addition to this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.