ALPK1 Rabbit mAb for WB, IHC-P, ELISA - E9QNX4

Core Product Specifications and Parameters

Biological Background: ALPK1 Function and Localization

• ALPK1 (Alpha-kinase 1) is a serine/threonine-protein kinase encoded by the Alpk1 gene in mice (UniProt entry: E9QNX4).
• It contains an alpha-kinase domain, part of a unique family of protein kinases with atypical substrate recognition.
• The protein is classified as a transferase (EC 2.7.11.-), catalyzing the transfer of phosphate groups to target substrates.
• UniProt annotates ALPK1 as part of the reference proteome for Mus musculus, indicating its conserved biological significance.
• The mouse ALPK1 protein has a predicted molecular weight of approximately 135.9 kDa (UniProt), though the recombinant protein construct may run at ~139 kDa in SDS-PAGE systems.
• Current UniProt record (unreviewed, TrEMBL) provides limited functional details; experimental characterization may reveal roles in innate immunity or cellular signaling.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 230-478 of mouse ALPK1, ensuring reactivity with the target's central region; verify specificity in your model system using appropriate controls.
• For Western blot, consider a starting dilution of 1:500-1:2000 and confirm the ~139 kDa band in mouse or rat tissue lysates.
• For IHC-P, optimize antigen retrieval (e.g., heat-mediated citrate buffer pH 6.0) and test signal-to-noise with known positive and negative tissue sections.
• In ELISA, the antibody can be used as capture or detection reagent; titrate against recombinant ALPK1 or native protein to establish assay conditions.
• Cross-reactivity with mouse and rat has been reported; reactivity with other species is not guaranteed and should be validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ALPK1 monoclonal antibody is suitable for research in kinase signaling, cell biology, and disease models, and we support scale-up and customization to meet your procurement needs. Inquire about bulk reagents, validated antibody pairs, and rare target sourcing to accelerate your assay development.