Alpha-Fetoprotein (AFP) Rabbit pAb - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein Function and Localization

• Alpha-fetoprotein (AFP), also known as alpha-1-fetoprotein or alpha-fetoglobulin, is a protein encoded by the AFP gene in humans.
• AFP is a secreted glycoprotein that belongs to the albuminoid gene superfamily and contains multiple intramolecular disulfide bonds, contributing to its structural stability.
• It is primarily synthesized by the fetal liver and yolk sac and is present in plasma; expression levels decline after birth.
• As a carrier protein, AFP binds copper, nickel, and fatty acids, and exhibits weaker binding to bilirubin, similar to serum albumin.
• Only a minor fraction (<2%) of human AFP demonstrates estrogen-binding properties.
• Post-translational modifications include glycosylation, phosphorylation, and sulfation, which may modulate its function and half-life.
• The mature protein has a predicted molecular weight of approximately 68.7 kDa and serves as a widely used biomarker in oncology and prenatal diagnostics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 450–550) of human AFP, which shares sequence conservation with mouse AFP, consistent with the reported cross-reactivity.
• For Western blotting (WB), consider optimizing antibody dilution to detect endogenous AFP in relevant biological samples (e.g., liver cell lysates or serum).
• For immunofluorescence/immunocytochemistry (IF/ICC), validate fixation and permeabilization conditions for your cell line, as AFP is a secreted protein.
• Ensure appropriate controls are included, particularly when analyzing samples with potential albumin cross-reactivity, given the structural similarity between AFP and serum albumin.
• When using ELISA, pair this antibody with a suitable detection antibody and optimize coating conditions for sensitive detection of AFP in plasma or serum.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers comprehensive sourcing solutions for diagnostic manufacturers and research labs. From this anti-AFP antibody to matched antibody pairs, ancillary reagents, and bulk custom manufacturing, we provide one-stop access to support your immunoassay development and cancer biomarker programs. Our supply chain covers rare targets and ensures timely delivery of quality-assured materials from concept to clinic.