Alpha-1 Antitrypsin (SERPINA1) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P01009

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antitrypsin Function and Localization

• Alpha-1-antitrypsin (SERPINA1), also known as alpha-1 protease inhibitor, alpha-1-antiproteinase, and serpin A1, is a member of the serpin superfamily.
• It acts as an inhibitor of serine proteases, primarily targeting elastase, with moderate affinity for plasmin and thrombin, and irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator.
• An aberrant form of the protein inhibits insulin-induced NO synthesis in platelets, decreases coagulation time, and has proteolytic activity against insulin and plasmin.
• SERPINA1 is a reversible chymotrypsin inhibitor that also inhibits elastase but not trypsin; a major physiological role is protecting the lower respiratory tract against proteolytic destruction by human leukocyte elastase.
• The protein is secreted and found in the endoplasmic reticulum, extracellular space, and extracellular matrix.
• It is ubiquitously expressed, with notable presence in leukocytes and plasma.
• Key functional and structural features include involvement in acute phase response, blood coagulation, hemostasis, and alternative splicing; it is a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• In Western blot, a band at approximately 47 kDa (theoretical MW 46.7 kDa) is expected; the immunogen corresponds to amino acids 25–315 of the mature protein, so the antibody may recognize both secreted and intracellular forms.
• For immunofluorescence/immunocytochemistry, consider permeabilization steps to detect intracellular pools localized in the endoplasmic reticulum; secreted and extracellular matrix-associated forms may require alternative fixation methods.
• The reported cross-reactivity with mouse and rat allows for cross-species studies, but validation in the specific experimental system is recommended.
• When used in ELISA, this polyclonal antibody can serve as a capture or detection reagent; pairing with another anti-SERPINA1 antibody may enhance assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering this high-quality rabbit polyclonal antibody against Alpha-1 Antitrypsin (SERPINA1) for respiratory disease and hemostasis research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—all supported by expert technical guidance from concept to clinic.