Alpha-1 Antichymotrypsin (SERPINA3) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01011

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antichymotrypsin Function and Localization

• Alpha-1-antichymotrypsin (SERPINA3) is a secreted serine protease inhibitor (serpin) belonging to the serpin superfamily.
• It inhibits neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
• Also known as cell growth-inhibiting gene 24/25 protein, it is encoded by the SERPINA3 gene and may play a role in cell growth regulation.
• Synthesized in the liver and secreted into plasma, its concentration rises sharply during the acute phase of inflammation or infection.
• Alpha-1-antichymotrypsin is a glycoprotein with post-translational modifications including glycosylation and a cleaved signal peptide.
• It has been identified in the amyloid plaques from the hippocampus of Alzheimer disease brains, suggesting involvement in neurodegenerative pathology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 24-423, covering nearly the entire mature protein; the resulting polyclonal antibody likely recognizes multiple epitopes, suitable for detecting both native and denatured forms.
• In WB, expect a band approximately at 48 kDa corresponding to the glycosylated alpha-1-antichymotrypsin; include appropriate positive controls (e.g., human plasma or liver lysate).
• For IHC-P, antigen retrieval steps may be required; consider testing on formalin-fixed paraffin-embedded liver or inflamed tissue sections, and validate with appropriate controls.
• In ELISA applications, this antibody can serve as capture or detection reagent; the target being secreted, analyze serum or plasma samples, and pair with a second antibody for sandwich format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This alpha-1-antichymotrypsin antibody supports research in serpin biology, acute phase response, and Alzheimer's disease. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development.