ALK Rabbit mAb for WB, IHC-P, ELISA - Q9UM73

Core Product Specifications and Parameters

Biological Background: ALK Function and Localization

• ALK (Anaplastic lymphoma kinase) is a receptor tyrosine kinase that belongs to the insulin receptor superfamily.
• It is essentially and transiently expressed in specific regions of the central and peripheral nervous systems, playing a critical role in the genesis and differentiation of the nervous system. Related references: PMID:11121404, PMID:11387242, PMID:16317043
• ALK functions as a key regulator of energy homeostasis by controlling energy expenditure and acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone in hypothalamic neurons.
• Ligand binding (primarily ALKAL2) activates ALK and triggers downstream signaling through the MAPK pathway, leading to cellular responses such as proliferation and differentiation. Related references: PMID:30061385, PMID:33411331, PMID:34646012, PMID:34819673
• ALK also interacts with pleiotrophin (PTN) and midkine (MDK), which induce MAPK pathway activation, anti-apoptotic signaling, and cell proliferation. Related references: PMID:11278720, PMID:11809760, PMID:12107166, PMID:12122009
• Signaling through ALK leads to tyrosine phosphorylation of substrates like IRS1, SHC1, and FRS2, and activation of downstream effectors including PI3K/AKT, MAPK1/ERK2, MAPK3/ERK1, and NF-kappa-B, promoting cell growth and survival. Related references: PMID:15226403, PMID:16878150
• Subcellularly, ALK is localized at the cell membrane.
• Expression is observed primarily in the brain, CNS, and at lower levels in the small intestine and testis, but not in normal lymphoid cells.
• ALK is subject to post-translational modifications including glycosylation and phosphorylation, and its gene is associated with chromosomal rearrangements in various cancers, making it a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular kinase domain of ALK (aa 1093-1411), suggesting the antibody is suitable for detecting total ALK protein.
• For Western blot, the predicted molecular weight of ALK is ~176 kDa; ensure appropriate gel percentage (e.g., 6-8% SDS-PAGE) and transfer conditions for high-molecular-weight proteins.
• For IHC-P, validate antibody specificity in ALK-expressing tissues such as brain or testis sections, and consider using a blocking peptide or negative control to confirm staining.
• For ELISA, optimize capture and detection conditions using recombinant ALK protein as a standard.
• Cross-reactivity is reported for human; validation in other species should be performed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, including this ALK monoclonal antibody for applications such as cancer biomarker detection and neuroscience research. With a comprehensive supply of antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we support your assay development from concept to clinic. Explore our rare-target sourcing capabilities for your full ALK signaling panel needs.